Creative biogene

For over a decade, the QVirus Platform at Creative Biogene has optimized our baculovirus production systems. We offer a comprehensive portfolio of products and services for the efficient design, development, production, and analytical testing of baculovirus vectors. https://qvirus.creative-biogene.com/servic/baculovirus.html

Creative Biogene’s experienced experts can help you synthesize high-quality CpG oligodeoxynucleotides, including type A ODNs, type B ODNs, and type C ODNs. https://www.creative-biogene.com//cpg-oligodeoxynucleotide

Target region sequencing is a highly targeted approach for analyzing genetic variation in specific genomic regions. The ultra-deep sequencing of PCR products (amplicons) allows efficient variant identification and characterization. Combining genome-wide off-target detection methods (SITE-seq, GUIDE-seq, WGS) allows the true on-target/off-target mutations to be determined in genome editing experiments with high sensitivity and in a non-biased manner. https://www.creative-biogene.com//target-region-sequencing

To establish an immortalized cell line, foreign DNA needs to be delivered into target cells. However, transfection of many primary cells is challenging. Replication-deficient lentiviruses are often used as an efficient gene delivery tool during immortalization. Creative Biogene offers a series of lentiviral particles containing genes, including SV40 T, human TERT, and HPV-16 E6/E7, to generate immortalized cell lines. https://www.creative-biogene.com//cell-immortalization-len

CRISPR/Cas9 has been widely utilized to facilitate functional knockout of protein-coding genes. Creative Biogene is experienced in producing a fragment deleted cell line using any mammalian cell line and targeting sequences. The utilization of two sgRNAs targeting the immediate sequence upstream and downstream is a viable method for a target protein’s complete functional knockout. https://www.creative-biogene.com//fragment-deleted-stable-